Amplicon Sequence Alignment
The image above indicates the start mapping position (1st bp mapped) of each amplicon mapped read included in the experiment. This plot immediately tells the reviewer what site in the viral genome the reads are mapping to, and how many. With background knowledge of the expected primer anealing sites, expected read depth and false positive rate a knowledgable reviewer should instantly know if the alignments indicate a positive or negative COVID-19 result.
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Read Alignment Quantification
The mapping quality of reads by contig can tell us how accurately our amplified viral sequences are matching the COVID-19 genome. The highest achievable MAQ in Bowtie2 is 42. High numbers of mapping reads on multiple amplicons indicate a positive diagnosis for COVID-19, whereas low mapping at almost all will indicate a negative result (i.e. no COVID-19 detected).
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i = 0;
while (!deck.isInOrder()) {
print 'Iteration ' + i;
deck.shuffle();
i++;
}
print 'It took ' + i + ' iterations to sort the deck.';
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19.99 |
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Description |
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Item One |
Ante turpis integer aliquet porttitor. |
29.99 |
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Vis ac commodo adipiscing arcu aliquet. |
19.99 |
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Morbi faucibus arcu accumsan lorem. |
29.99 |
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Vitae integer tempus condimentum. |
19.99 |
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Ante turpis integer aliquet porttitor. |
29.99 |
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100.00 |
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